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Journal of Oral & Facial Pain and Headache (OFPH) is published by MRE Press from Volume 38 lssue 1 (2024). Previous articles were published by another publisher on a subscription basis, and they are hosted by MRE Press on www.jofph.com as a courtesy and upon agreement with Journal of Oral & Facial Pain and Headache.

Original Research

Open Access

Participation of Microglial p38 MAPK in Formalin-Induced Temporomandibular Joint Nociception in Rats

  • Kyoung A. Won1
  • Young M. Kang1,2
  • Min K. Lee3
  • Min Kyoung Park1
  • Jin S. Ju1
  • Yong C. Bae1
  • Dong K. Ahn1,2,*,

1Department of Oral Physiology, Daegu, Korea

2School of Dentistry, Kyungpook National University, Daegu, Korea

3Department of Dental Hygiene, Youngsan University, Yangsan, South Korea

DOI: 10.11607/jofph.26132 Vol.26,Issue 2,June 2012 pp.132-141

Published: 30 June 2012

*Corresponding Author(s): Dong K. Ahn E-mail: dkahn@knu.ac.kr

Abstract

Aims: To investigate nociceptive behavior and the immunoreactivity of microglia and phosphorylated-p38 (p-p38) mitogen-activated protein kinase (MAPK) following intracisternal administration of SB203580, a p38 MAPK inhibitor, or minocycline, a microglia inhibitor, in rats with temporomandibular joint (TMJ) inflammation. Methods: The number of nociceptive behavioral responses was recorded for nine successive 5-minute intervals following formalin injections into the left TMJ. SB203580 or minocycline was administered intracisternally 2 hours prior to the formalin injection. Statistical analysis used one-way analysis of variance followed by least significant difference post-hoc analysis. Results: The intra-articular injection of formalin increased the expression of p-p38 MAPK in the ipsilateral medullary dorsal horn. Most of the p-p38 MAPK co-localized with OX42, a microglial marker, but not with GFAP, an astrocyte marker. Intracisternal injections of SB203580 (0.5, 1, or 5 Μg) attenuated the number of nociceptive behavioral responses and the expression of p-p38 MAPK in the medullary dorsal horn. Intracisternal injections of minocycline (25 or 50 Μg) also attenuated the responses and the expression of OX42 and p-p38 MAPK in the medullary dorsal horn. Conclusion: These findings suggest that p38 MAPK in microglia plays an important role in the central processing of inflammatory TMJ nociception in rats. The data further indicate that a targeted blockade of the microglial p38 MAPK pathway is a potentially important new treatment strategy for inflammatory TMJ nociception.


Keywords

formalin; microglia; p38 MAPK; pain; TMJ


Cite and Share

Kyoung A. Won, Young M. Kang, Min K. Lee, Min Kyoung Park, Jin S. Ju, Yong C. Bae, Dong K. Ahn. Participation of Microglial p38 MAPK in Formalin-Induced Temporomandibular Joint Nociception in Rats. Journal of Oral & Facial Pain and Headache. 2012. 26(2);132-141.

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